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KMID : 0644120060190020162
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Journal of Oriental Gynecology
2006 Volume.19 No. 2 p.162 ~ p.185
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Effects of Gamigwichulpajing-Tang on the Development of Experimentally-induced Endometriosis in Rats
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Kim Yeu-Tae
Lee Dong-Nyung
Kim Hyung-Jun
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Abstract
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Purpose : This study was carried out to investigate the effects of Gamigwichulpajing-Tang(GGT) on the development of experimentally-induced endometriosis in rats.
Materials and Methods : Endometriosis was induced in rats by auotransplanting uterine tissue to the peritoneum and devided them into three groups: (1) sham-operated group(n=8), (2) surgically induced endometriosis and untreated control group(n=8), (3)surgically induced endometriosis and GGT treated group. GGT(700mg/head) was orally administrated for 15days after operation. Then we measured the body weight, the volumes of endometriotic implants, the weight of uterus and ovary, and investigated the content of cytokines(MCP-1, TNF-{alpha}, IL-l{beta}) in serum and ascites. Histopathology, immunohistochemistry for COX-2, and histochemistry for mast cell in transplanted uterine tissue were performed.
Results :- The volume(mm^3) of endometriotic implants in GGT-treated group(53.50;{pm}18.63) was significantly decreased(p<<0.01) compared with control group(404.50{pm}317.68). - The content(pg/ml) of MCP-1 in ascites in GGT-treated group(4265{pm}108) was significantly decreased(p<<0.001) compared with control group(8632{pm}1245). - The content(pg/ml) of TNF-{alpha} in serum in GGT-treated group(64.5{pm}21.6) was significantly decreased(p<<0.05) compared with control group(147.1{pm}78.2). - The content(pg/ml)- of TNF-{alpha} in ascites in GGT-treated group(738.3{pm}502.4) was significantly decreased(p<<0.05) compared with control group(1245.2{pm}362.2). - The percentage(%) of positive epithelial layers for COX-2 in GGT-treated group(22.9{pm}9.3) was significantly decreased(p<<0.001) compared with control group(50.2{pm}8.2). - The number of mast cells in adjacent tissue of transplanted uterine tissue in GGT-treated group(61.4{pm}13.9) was significantly decreased(p<<0.001) compared with control group(109.3{pm}30.2). - The number of mast cells in stroma of transplanted uterine tissue in GGT-treated group(9.4{pm}2.7) was significantly decreased(p<<0.001) compared with control group(26.0{pm}7.7). - Histopathologically, proliferation of endomeuiotic epithelia and stroma, and infiltration of inflammatory cells in transplanted uterine tissue of GGT-treated group were weakly observed than those of control group.
Conclusion :On the basis of these results, we concluded that Gamigwichulpajing-Tang have inhibiting effects on the development of transplanted uterine tissue. And these effects may be related with decreased Production of MCP-1 and TNF-{alpha}, decreased expression of COX-2, and decreased infiltration of mast cells by administration of Gamigwichulpajing-Tang.
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KEYWORD
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Endometriosis, Gamigwichulpajing-Tang, MCP-1, TNF-{alpha}, 1L-l{beta}, COX-2, Mast cell
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